William N Green
Research Summary / Selected Publications
My research is focused on the cell biology of ionotropic neurotransmitter receptors, the receptors responsible for the rapid postsynaptic response in nerve and muscle. These receptors are large oligomeric membrane proteins with subunits surrounding an ion channel that opens when neurotransmitters bind to the receptor. There are two different families of ionotropic neurotransmitter receptors. One family includes nicotinic acetylcholine receptors (AChRs), GABA and glycine receptors, and the other family covers the ionotropic glutamate receptors. There are several projects ongoing in my lab characterizing the assembly, trafficking and clustering of these receptors. Assembly refers to the processes that transform newly synthesized subunits into functional receptors usually in the endoplasmic reticulum. Trafficking refers to the processes that transport the receptors to different location in cells and targets them to these locations. Clustering are the processes that pack and maintain the receptors in regions of high density such as synapses.
My research is focused on the cell biology of ionotropic neurotransmitter receptors, the receptors responsible for the rapid postsynaptic response in nerve and muscle. These receptors are large oligomeric membrane proteins with subunits surrounding an ion channel that opens when neurotransmitters bind to the receptor. There are two different families of ionotropic neurotransmitter receptors. One family includes nicotinic acetylcholine receptors (AChRs), GABA and glycine receptors, and the other family covers the ionotropic glutamate receptors. There are several projects ongoing in my lab characterizing the assembly, trafficking and clustering of these receptors. Assembly refers to the processes that transform newly synthesized subunits into functional receptors usually in the endoplasmic reticulum. Trafficking refers to the processes that transport the receptors to different location in cells and targets them to these locations. Clustering are the processes that pack and maintain the receptors in regions of high density such as synapses.
Singaraja RR, Kang MH, Vaid K, Sanders S, Vilas G, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini AE, Green WN, Berthiaume L, Hayden MR. 2009.
Palmitoylation of ATP-Binding Cassette Transporter A1 Is Essential for Its Trafficking and Function. Circulation Research 105: 138-47.
Govind AP, Vezina P, Green WN. 2009.
Nicotine-induced upregulation of nicotinic receptors: Underlying mechanisms and relevance to nicotine addiction. Biochemistry Pharmacology 78: 756-65.
Waites CL, Specht CG, Härtel K, Leal-Ortiz S, Genoux D, Li D, Drisdel RC, Jeyifous O, Cheyne JE, Green WN, Montgomery JM, Garner CC. 2009. Synaptic SAP97 isoforms regulate AMPA receptor dynamics and access to presynaptic glutamate.
Journal of Neuroscience 29: 4332-45.
Kang R, Wan J, Arstikaitis P, Takahashi H, Huang K, Bailey AO, Thompson JX, Roth AF, Drisdel RC, Mastro R, Green WN, Yates JR 3rd, Davis NG, El-Husseini A. 2008. Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.
Nature 18: 904-9.
Christianson, J. C. and W. N. Green. 2004. Regulation of Nicotinic Receptor Expression by the Ubiquitin-Proteasome System. EMBO Journal 23:4156-65.
Drisdel, R. C., E. Manzana and W.N. Green. 2004. The role of palmitoylation in functional expression of nicotinic 7 receptors Journal of Neuroscience 24:10502-10510.
Mitra, M., C. P. Wanamaker, J. C. Christianson and W. N. Green. 2001. Rearrangement of nicotinc receptor subunits during the formation of ligand binding sites. Journal of Neuroscience 21: 3000-3008.
Drisdel, R. C. and W.N. Green. 2000. Neuronal -bungarotoxin receptors are homomers composed of five 7 subunits. Journal of Neuroscience 20: 133-139.
Eertmoed, A. L. and W. N. Green. 1999. Nicotinic receptor assembly requires multiple regions throughout the gamma subunit. Journal of Neuroscience 19: 6298-6308.
Rakhilin, S., R. C. Drisdel, D. Sagher, D. McGehee, Y. Vallejo and W. N.Green. 1999. - Bungarotoxin receptors contain 7 subunits in two different disulfide-bonded conformations. Journal of Cell Biology 146: 203-218.
Green, W. N. and T. Claudio. 1993. Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially. Cell 74: 57-69.
Green, W. N. and C. P. Wanamaker. 1998. Formation of the nicotinic acetylcholine receptor binding sites. Journal of Neuroscience 18:5555-5564.
Singaraja RR, Kang MH, Vaid K, Sanders S, Vilas G, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini AE, Green WN, Berthiaume L, Hayden MR. 2009.
Palmitoylation of ATP-Binding Cassette Transporter A1 Is Essential for Its Trafficking and Function. Circulation Research 105: 138-47.
Govind AP, Vezina P, Green WN. 2009.
Nicotine-induced upregulation of nicotinic receptors: Underlying mechanisms and relevance to nicotine addiction. Biochemistry Pharmacology 78: 756-65.
Waites CL, Specht CG, Härtel K, Leal-Ortiz S, Genoux D, Li D, Drisdel RC, Jeyifous O, Cheyne JE, Green WN, Montgomery JM, Garner CC. 2009. Synaptic SAP97 isoforms regulate AMPA receptor dynamics and access to presynaptic glutamate.
Journal of Neuroscience 29: 4332-45.
Kang R, Wan J, Arstikaitis P, Takahashi H, Huang K, Bailey AO, Thompson JX, Roth AF, Drisdel RC, Mastro R, Green WN, Yates JR 3rd, Davis NG, El-Husseini A. 2008. Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.
Nature 18: 904-9.
Christianson, J. C. and W. N. Green. 2004. Regulation of Nicotinic Receptor Expression by the Ubiquitin-Proteasome System. EMBO Journal 23:4156-65.
